Course Title:General Microbiology
Course Number: BIO. 207.01
Credit Hours: Four (4) Semester hours
Pre-requisites: BIO. 101 and BIO. 102
Class Meetings: M,W,F, 9:00 - 9:50 AM
Lecture Hall: Grim Hall, 211
Laboratories: BIO. 277.01 & .02
Instructor: J. Abassi
BIO. 277.01 T, 1:00 Š 3:50 PM
BIO. 277.02 W, 1:00 Š 3:50 PM
Grim Hall, 213
Textbooks:
1) Microbiology (seventh edition). G.J. Tortora, B. R.
Funke and C.L. Case. 2000. Benjamin Cummings.
2) Microbiology: A laboratory manual (fifth edition). J.G.
Cappuccino and N. Sherman. 1998. Benjamin Cummings.
Other materials: Notebooks for lectures and laboratory reports.
Course Description
This course is designed to meet the requirements of the Biology
DepartmentÕs curriculum which makes General Microbiology compulsory
for all Biology majors. It is also designed to meet the needs
of Pre-Nursing majors who want to take an introductory Microbiology
course.
General Microbiology is a one semester course that introduces
students to the importance and applications of Microbiology in
food production, industry, environment and human, veterinary and
plant health. The mechanisms of pathogenicity of harmful organisms
as well as methods for their control will be explored. Students
will be introduced to methods of cultivating microbes including
nutritional and environmental needs of some common microbes. Identification
of common microbes by macroscopy, microscopy, morphology, biochemical
reactions and serology will also be explored.
General Microbiology is complimented by laboratory exercises
in which students acquire hands on experience in studying various
aspects of microbiological applications.
Course Goals: Upon completion of the course, students should
be able to:
a) appreciate the scope and career opportunities in Microbiology,
b) understand the principles and applications of microscopes,
c) understand the anatomy and physiology of the bacterial cell,
d) understand the genetics, growth and nutrition of microbes,
e) discuss the principles and applications of primary and secondary
immune responses, f) discuss the principles of the physical and
chemical control of microbes, g) understand the role of microbes
as agents of infectious diseases as well as mechanisms of pathogenesis
and control of infection, h) discuss the control of nosocomial
infections in health care facilities, i) initiate projects in
epidemiology of microbial infections, j) study microbes in food,
water, sewage and the environment.
Course Requirements
a) Tests: There will be tests and quizzes covering lectures as
well as textbook reading assignments, plus a mid-term and final
examination. There will be four announced tests and four unannounced
quizzes per semester. Cheating during tests, quizzes and examination
is a university offense. Students will get an F (failing) grade
for the test, quiz or examination and will be reported to the
Office of the Registrar if the offense is repeated (see the Academic
Integrity Statement).
b) Attendance: This is mandatory as there will be no make up
quizzes. A daily official class attendance record will be maintained
and become part of each studentÕs record. Students will be penalized
for more than three absences. Late coming, disruptive behavior
including noise making will not be tolerated. Up to three (3)
absences will not be penalized. From four (4) to six (6) absences,
the penalty is 5% of final grade and seven (7) and eight (8) absences
will attract a penalty of 10% of final grade. From nine (9) absences,
the penalty is a failing grade.
c) Laboratory exercises: Students taking General Microbiology
are also to take the laboratory class, BIO. 277.01 or BIO. 277.02.
The laboratory exercises account for 25% of the total marks awarded.
Students will be required to wear protective clothing during laboratory
exercises. Laboratory reports must be typed and submitted no later
than seven (7) days after completion of the exercise. No laboratory
make-ups will be done. No laboratory reports will be accepted
from students who did not perform the exercise.
d) Assignments: There will be one assignment each before and
after mid-term. No late submissions will be accepted without prior
consultation and approval by the instructor.
e) Grading: The final grade shall be the average of grades earned
on quizzes, tests, assignments, laboratory reports, mid-term and
final examinations. The mid-term grade will account for 50% of
the total points.
| Laboratory
Work Schedule |
| Week
|
Topics |
Page |
| 1 |
a) Safety
precautions in the laboratory.
b) Care and use of the light microscope. c) Principle and
use of the autoclave.
d) Writing laboratory reports.
|
|
| 2 |
a) Transfer
of microbial cultures (sub-culturing)
|
7 - 9 |
| 2 |
b)
Isolation of pure cultures (streak plate; spread plate; pour
plate) |
13
- 15 |
| 2 |
c)
Isolation of discrete colonies from mixed cultures |
16
- 18 |
| 3 |
Cultural
characteristics of microorganisms |
21 - 23 |
| 4 |
a)
Microscopy |
27
- 34 |
| 4 |
b)
Hanging drop preparation |
37
- 38 |
| 5 |
a)
Principles of staining bacteria |
45 - 47 |
| 5 |
b)
Preparation of bacterial smears |
49
- 51 |
| 5 |
c)
Simple staining techniques |
55
- 57 |
| 5 |
d)
Negative staining techniques |
59 - 61 |
| 6 |
a)
Gram staining |
63
- 67 |
| 6 |
b)
Acid fast staining |
69 - 71 |
| 7 |
a)
Spore staining |
75
- 77 |
| 7 |
b)
Capsule staining |
79
- 81 |
| 8 |
a)
Cultivation of microorganisms |
83
- 85 |
| 8 |
b)
Defined; Artificial media |
87
- 88 |
| 8 |
c)
Differential; Selective; Enriched; Enrichment media |
93
- 97 |
|
9 |
Physical factors affecting growth of microbes |
|
| 9 |
a)
Temperature |
99 - 101 |
| 9 |
b)
pH of medium |
103
- 105 |
| 9 |
c)
Oxygen requirement |
107
- 111 |
| 10 |
a)
Quantitation (enumeration) of viable bacterial cells |
119
- 123 |
| 10 |
b) Bacterial growth curve |
125
- 131 |
| 11 |
a)
Biochemical activities of bacteria |
133
- 137 |
| 11 |
b) Carbohydrate fermentation |
141
- 145 |
| 11 |
c)
IMVIC tests |
151
- 155 |
| 11 |
d)
Catalase test |
179
- 181 |
| 12 |
a)
Cultivation of molds |
203
- 206 |
| 12 |
b) Yeast morphology |
209
- 211 |
| 12 |
c)
Identification of unknown fungi |
213
- 215 |
| 13 |
a)
Physical and chemical agents for the control of microbial growth |
235 - 250 |
| 13 |
b) Chemotherapeutic agents |
253
- 259 |
| 14 |
a)
Microbiology of water |
295-305
|
